Name: ArthroCell™ Cellular Bone Allograft: Cellular Component Overview
Uploaded: 2016-07-06T00:00:00Z
Channel: Arthrex

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VPT1-00643-EN | A | ArthroCell™ Cellular Bone Allograft: Cellular Component Overview

Thomas Temple

Human

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ArthroCell™ Cellular Bone Allograft: Cellular Component Overview

My name is Thomas Temple and I'm an orthopedic oncologist in Miami, Florida. Today I'm going to talk about the bone allogeneic cell product. The cells that we chose for this product are osteoprogenitor cells that are derived from the bone matrix, as well as the Miami cell, which is a marrow isolated adult multilineage inducible cell. These cells are unique insofar as they express the surface marker ss four. On this video, you could see these cells communicate with one another in in culture and exchange genetic information. These cells are primitive stem cells and cross germ layers. And you can see that depending on the matrix in which they find themselves, they can produce bone, they can produce nerve, even hepatocytes. When we look at the microarray and the gene expression of the Miami cell, it's interesting that it expresses many of the same genes that are seen in embryonic stem cells. And when we compare three different lines of cells, the Miami cell, the embryonic cell and bone marrow aspirates, you can see that the crossover in gene expression is quite significant between the Miami cell and the And the Embryonal cell. Furthermore, the Miami cell does not express MHC 1 or 2 antigens. Therefore, it is not recognized by the host. In preparation of these cells, we remove bone aseptically and then immediately morceli's the bone and place it in a shaker. The supernatant produced from this is poured off into a fecal gradient, and the mononuclear layer is then isolated and transferred into a selective low O2 media. In this fashion, we can select the Miami cells as well as the osteoprogenitor cells that seem to survive very well in a hypoxic environment, to the exclusion of other unwanted mononuclear cells such as red blood cells, white cells, T lymphocytes and macrophages. So in summary, the Miami and progenitor MSCs are selected and controlled in preparation of this product. The progenitor MSCs express stem cell markers, specifically Cd150 and Stro one, whereas the Miami cells are selected based on the expression of the SC four marker. These cells are highly inducible, and they share many of the genes expressed by embryonic stem cells and do not express the MHC 1 or 2 antigens.

ArthroCell™ Cellular Bone Allograft: Cellular Component Overview

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