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VID1-01462-EN | A | ArthroCell™ Allograft

Chelsea Day

Human

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ArthroCell™ Allograft

Today we will be demonstrating the proper preparation of ArthroCell. Arthrocell is our viable cellular bone graft, comprised of three components a micro particulate derived from cortical and cancellous bone, a gel component made of type one collagen from cortical bone, and a cell component derived from donor vertebrae. These three components come together to provide a bone graft, that is, osteoinductive, osteoconductive, and osteogenic, all the essential elements needed for bone healing. All of the ArthroCell components will arrive frozen in a sealed pouch. These products should be transferred onto the sterile field when transferring ArthroCell to the sterile field. Please note that the individual chevron pouches are not sterile. These individual chevron pouches should be opened utilizing aseptic technique, and only the pouches inside each chevron pouch can be presented to the operative field. The cell vial and the gel container need to be thawed in a room temperature. Sterile saline or sterile water bath. The cell vial takes about five minutes to thaw, while the gel component needs more time, about 20 minutes. While the cell vial and the gel container are thawing, add the recommended saline volume directly to the bone particulate. Once the cell vial has thawed, remove the vial from the saline bath. The cell vial can be added to the micro particulate container and mixed with the bone using a spatula. Replace the cap back on the micro particulate and set aside for at least ten minutes. We are now ready to prepare the gel component of our ArthroCell graft. The gel component gives the graft the hydrophobic and cohesive characteristics, allowing the graft to be moldable and resist irrigation. You can see that the gel is pretty thick at this point. To turn this into a paste like consistency, we will divide the gel into four pieces with our spatula and transfer the pieces into the abs. 2000 Mixing Syringe. Unsnap the push rod from the mixing element and mix the gel for about 60s. You will want to make sure to push and pull the entire length of the syringe to ensure no gel pieces are left intact. Once the gel component is at the desired consistency, you can snap the pushrod back into the mixing element and remove the syringe cap. The gel is now ready to be added to the cell particulate mixture. Using a spatula. You can mix the three components together until well incorporated. You can now transfer the matrix into a gloved hand for further molding until the desired graft configuration is obtained. At this point, the ArthroCell graft is ready for implantation. If the surgeon is not ready for the graft at this time, the graph can be stored capped in the container at room temperature until the graft is needed. However, keep in mind that the total time between graft thaw and implantation should not exceed four hours.

ArthroCell™ Allograft

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