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VID1-01030-EN | A | Angel® System - Creating a Clot with PRP or BMC

Robert Harrison

Human

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Angel® System - Creating a Clot with PRP or BMC

To make a gelling solution to clot PRP or bone marrow concentrate, you will need 5000 units of bovine thrombin and 10% calcium chloride solution. When you open the thrombin package, you will notice that there is a vial of saline and the thrombin powder. Please discard the sodium chloride solution vial. This will not be required as we will reconstitute the bovine thrombin powder with calcium chloride. Calcium chloride is required because aicda has been used to prepare the PRP and bone marrow concentrate. Aicda binds calcium. Therefore, to create a stable clot, we will need to add calcium back to the gelling solution. For every 5000 units of bovine thrombin or recombinant human thrombin, you will need one milliliter of calcium chloride 10% solution. In this example, we have 5000 units, so we will need five milliliters of calcium chloride. 10% solution to reconstitute the thrombin powder. Remove the cap utilizing aseptic technique. Add the calcium chloride to the thrombin vial. Now that we have our gelling solution prepared, we will utilize the high viscosity Viscous-Gel applicator to create a clot. The ratio of gelling solution to PRP and bone marrow concentrate is 1 to 10. Here we have a one cc syringe and a 12 cc syringe. The gelling solution goes in the one cc syringe. Once we have our gelling solution loaded in the one cc syringe and the PRP or bone marrow concentrate loaded in the 12 cc syringe, we are ready to attach the Viscous-Gel applicator tip. It is a Luer lock fit and is easily attached to the applicator. As the gelling solution and the PRP are expressed, the PRP or bone marrow concentrate will become activated and a clot will form. This clot takes approximately 30s to form. A stable clot has been formed.

Angel® System - Creating a Clot with PRP or BMC

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