Orthobiologics

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VID1-01239-EN | A | Thrombinator™ System

Robert Harrison

Human

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Thrombinator™ System

You will need whole blood or platelet poor plasma, calcium chloride and the Thrombinator system device. When using platelet poor plasma that has been prepared using a CDA solution, you will need to add calcium chloride to reverse the CDA. Step one is to introduce the calcium chloride into the Thrombinator system. In the port that is marked, inject 0.1ml of 10%. Calcium chloride solution will be injected into the port. Next you will introduce four milliliters of platelet poor plasma. You will need to mix for five seconds. Then place flat. The incubation time is ten minutes. You now have a stabilized clot. We will need to break this clot up by shaking vigorously. We have now disrupted the clot and will introduce our calcium chloride and platelet poor plasma through the injection port. We will also at this time, place our filter on the withdrawal port and shake again. For five seconds. We will place flat and wait for one minute. After one minute you will again have a clot. We will now disrupt this clot by shaking vigorously and withdraw our autologous serum. Uncap the filter. Attach a syringe. Invert and withdraw your autologous serum. To create a clot. You will need a ratio applicator. We are using a 1 to 1 ratio applicator. You will see on the right side of the applicator I have placed the autologous serum. I will now place next to it the platelet poor plasma for instructional purposes. We have the ratio applicator assembled and will now produce a clot. You should see a stable clot formation within 8 to 15 seconds.

Thrombinator™ System

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